Which Instrument is the best suited for my research?
The Monolith NT.115 and the Monolith NT.LabelFree complement each other well. Each system has its specific advantages and a decision has to consider your specific applications. If you are interested in measuring a wide variety of different samples, we recommend considering the use of both systems.
| NT.115: Measuring with fluorescence label: |
NT.LabelFree: Measuring with intrinsic fluorescence: |
|---|---|
| You can measure in any buffer and also in complex bio-liquids like cell lysate. | You avoid attaching a label to a sample and can thus measure also samples that might be sensitive to a labeling procedure (e.g. some membrane receptors). |
| MST with dye fluorescence allows measuring a wide range of binding events, from ion binding to ribosome or histone binding, as well as multi-component interactions. | The system is extremely sensitive, even for slightest modification of your molecule of interest. |
| You can measure any interaction of (bio)molecules independent of the respective spectroscopic properties of the titrated binding partner. | The system works perfectly for all systems where the titrated binding partner shows no or only a weak intrinsic fluorescence. That includes nucleic acids, peptides, sugars, most small molecules and many proteins. |
| You can measure an affinity range from sub-nM to mM. | You can measure an affinity range from about 10nM to mM. |
- NT.115
- NT.LabelFree
For further information, please contact us.