NT006 - Protein-Ion Interaction Analysis
Binding of Calcium Ions to Synaptotagmin
measured with fluorescence label and label-free
The synaptic vesicle protein synaptotagmin 1 is the main calcium sensor of neuronal exocytosis. Calcium binds to its cytosolic portion that consists of tandem C2-type domains. In this work we show that Microscale Thermophoresis is a valuable tool to measure binding of ions to proteins, with and without the use of a fluorescent label.
