Matrix Signaling in Inflammation and Fibrosis

Prof. Dr. med. Liliana Schaefer, Pharmazentrum /ZAFES, Frankfurt a.M., Germany

"The work of our group gave rise to the novel concept that under certain conditions matrix components may act as endogenous "danger" signals, which are recognized by innate immunity receptors, and are capable to trigger an inflammatory response reaction. We are using MST to measure the interactions of endogenous and in vitro mutagenized proteoglycans of the extracellular matrix with their receptors and are very satisfied with the results obtained so far. The affinities are consistent with the biological readouts and confirm previous results gained with immunprecipitation and binding assays. MST is a new technology we can definitely recommend for obtaining robust quantitative affinity data."

Prof. Liliana Schäfer