This category refers to applications that determine the number of binding sites on a molecule.

This category refers to applications that determine the number of binding sites on a molecule. These application require a different experimental setting. Here, the molecule which is kept constant is used at a concentration, that is well above the dissociation constant of the interaction. A binding partner is titrated in. The molecular ratio, at which the saturation of binding sites is reached yields the number of binding sites and/or activity of the protein preparation.